THE belief that at least some passerine birds reject nonmimetic eggs
placed in their nests was confirmed long ago by the experiments of Swyn-
nerto.n (1916, 1918) and Rensch (1924). The latter worker (Rensch
1925) went on to question whether such birds actually recognize their
own eggs (rejection by true egg recognition) or whether they simply reject
any egg that differs from the majority (rejection by discordancy). Rensch
was interested in egg recognition because he believed it to be vital to the
evolutionary interactions between brood parasites and their hosts. Whether
birds rejected eggs on the basis of true recognition or discordancy, the
behavior would still function as an efficient antiparasite adaptation because
brood parasites generally deposit only one egg in each host nest. Although
Rensch's (1925) experiments have been widely interpreted as demonstrat-
ing rejection by discordancy (e.g. Welty 1963), the bulk of his results
actually indicate true egg recognition (Rothstein 1970). Numerous re-
cent experiments and a literature review suggest that most or all passerines
that reject foreign eggs practice true egg recognition (Rothstein 1974).
Nearly all these recent experiments, though, were conducted on birds
that had completed their dutch. The fact that birds that reject foreign
eggs practice true egg recognition after completing their clutch prompts
two significant questions: (1) Is this recognition as highly developed at
earlier stages of the breeding cycle? (2) Which components of rejection
by true egg recognition are primarily learned and which are primarily
innate? This paper reports on experiments that were designed to deal
with these two questions.
The new experiments reported here were done on naturally breeding
Gray Catbirds (Dumetella carolinensis). Previous experiments showed
that catbirds are extremely intolerant of foreign eggs placed in their
nests. Single artificial or real cowbird eggs experimentally added to 30
catbird nests in eastern North America (Connecticut, Maryland, and
Michigan) were all removed by the catbirds. Catbirds in western North
America (Manitoba and Nebraska) may be slightly more tolerant, with
22 of 25 nests yielding ejections of single cowbird eggs. In 17 additional
catbird nests (from Connecticut and Maryland), where experiments re-
sulted in clutches consisting of only cowbird eggs or of only one catbird
egg and two or more cowbird or other type of foreign eggs, the catbirds
ejected the foreign eggs in every instance (see Rothstein 1974 for deta/ls).
Friedmann (1963) has stated that while such intolerance of foreign eggs
serves as an efficient host defense it is probably not evolved in response
to brood parasitism. I suggest this intolerance can be explained only in
terms of an adaptation that evolved because it protects birds from brood
parasites. This is the most parsimonious explanation, as rejecting foreign
eggs does not seem to have any adaptive value in most birds, other than
in the context of brood parasitism (Rothstein 1970, MS).
Although catbird and cowbird eggs differ little in size (averaging 23.3
x 17.5 mm and 21.45 x 16.42 mm, respectively, according to Bent 1948,
1958), they are strikingly different in coloration and thus provide a valu-
able pair for experimentation. Catbird eggs are immaculate with a blue-
green ground color whereas cowbird eggs usually have a whitish ground
color and are heavily mottled with brown and gray spots.
Previous work by Tschanz (1959) has shown that in a nonpasserine,
the Common Murre (Uria aalge), egg recognition is realized through a
learning process. In the case of a passerine, the Village Weaverbird
(Ploceus cucullatus), it has been suggested that egg recognition is also
learned (Victoria 1972). Given the variable nature of murre and weaver-
bird eggs these findings might not be unexpected. The eggs of most song-
birds, the catbird included, show little variation and egg recognition
through largely innate means might not be difficult to evolve. Never-
theless the hypothesis of egg recognition by learning will be supported
by a demonstration of greater tolerance toward a foreign egg type in the
early stages of the breeding cycle than in later stages. If egg recognition
has a learning component, greater tolerance in the early stages of the
nesting cycle would be expected whether the birds learn the appearance
of their eggs anew with each breeding attempt, or whether the learning
occurs only in completely naive individuals during the first nesting at-
tempt of their lives. In the latter case though, increased acceptance early
in the cycle would occur only at those nests tended by naive birds. Un-
fortunately it is impossible to determine whether the catbirds tested in
the experiments reported here were naive or experienced; but given the
high mortality of passerines it is reasonable to assume that within any
series of nests experimented upon in the field some will be tended by naive
and others by experienced individuals.
Much of what follows is relevant only to the catbird and to other
species that I have termed "rejecters." Experiments with cowbird eggs
have shown little intraspecific variation in response to artificial cowbird
parasitism. A minority of North American species reject cowbird eggs
at nearly 100% rates. These are the rejecters. Most species accept the
eggs at nearly 100% rates and are termed "accepters." Rejecter and
accepter species have presumably responded and not responded, respec-
tively, in a major way to the evolutionary pressures of brood parasites
(Rothstein 1970). As accepter species demonstrate little or no egg rec-
ognition (except under special circumstances (Rothstein 1970, MS)),
questions on their acquisition of this behavior have reduced relevancy.
}VETItODS AND CONTROLS FOR ARTIFICIAL EGGS
The artificial eggs used in most experiments were cast in plaster of paris and
painted with artist's paints of the acrylic polymer type. Artificial cowbird and
catbird eggs were also shellacked. I have elsewhere (Rothstein 1970, 1974) described
the production and properties of these eggs in greater detail. Suitable controls for
artificiality of the eggs have been performed. For example, at four nests all four
of the catbirds' own eggs were replaced with one artificial catbird egg plus three
artificial cowbird eggs. In all four cases the cowbird eggs were ejected while the
artificial catbird egg was left in the nest. At another five nests a single artificial
catbird egg added to a nest with two or more real catbird eggs was accepted, where-
as a single real or artificial cowbird egg was rejected under similar conditions at
52 of 55 nests from five localities in North America. These different acceptance
rates (5-o vs. 3-52) are significant at P % 0.005 (Fisher Exact Probability Test,
Siegel 1956). Throughout this paper data on which statistical tests have been per-
formed will be reported as in the preceding sentence. The entire two-by-two con-
tingency table can be constructed from the information given (e.g. 5-0 vs. 3-52).
To determine whether catbirds possess true egg recognition at an early stage in
their breeding cycle and whether any recognition that occurs is learned, the fol-
lowing manipulation (experiment I) was performed: The first egg laid in 11 catbird
nests was replaced with an artificial cowbird egg. The time interval between egg
laying and initiation of the experiment is unknown, but it could have ranged from
a few minutes to 4 h. The nests were checked later the same day and then the
next morning. In some cases additional egg manipulations were conducted. Of the
11 nests studied 10 were in New Haven County, Connecticut during May and June
1969 and 1970; the 11th nest, number 72-215, was in Anne Arundel County, Mary-
land in May 1972.
RESULTS FROM PRIMARY EXPERIMENTS
Results of experiment I are summarized in Table 1. At three nests,
the cowbird egg was missing by the same afternoon of the day it had
been inserted. At another three, it was missing and a second catbird egg
was in its place the next morning. The catbirds at these six nests clearly
recognized their own egg type even at the earliest stages of clutch initia-
tion. At the three nests where the cowbird egg was ejected by the same
afternoon, it was ejected in the absence of any cues or information that
one or more catbird eggs might have supplied. It cannot be determined
whether the cowbird eggs in the other three nests were ejected before or
after the second catbird egg was laid. But it is of course certain that
they were not the minority type egg at the time of their ejection. It could
be argued that the missing cowbird eggs in these six nests were removed
TABLE 1
RESPONSES OF CATBIRDS TO ARTItlCIAL COWBIRD EGGS PLACED IN TIIER NESTS'
Status of cowbird egg Status of cowbird egg
after 1 day after 2 days
Experimental procedure
and location Accepted Ejected Accepted Ejected
I First catbird egg laid replaced
with an artificial cowbird egg
(experiment done within 4 h
after the first egg was laid);
Connecticut, Maryland
II One artificial cowbird egg added
to clutch when there were at
least two catbird eggs present;
Connecticut, Maryland,
Michigan
5 6 4 7
4 25 2 27
x A catbird egg was removed at the time the cowbird egg was added in 23 of the 29 experiment
II nests. Whether or not a catbird egg is removed at the time of experimentation has no effect
on response (Rothstein 1970). At one experiment II nest a real cowbird egg was used rather than
an artificial one. It was ejected within a day.
by predators rather than by the catbirds, but 20 other catbird nests sub-
jected to different experimental procedures during the same years and
in the same study areas as the experiment I nests can serve as a control
for nest predation. Only one of these nests suffered nest predation (all
the eggs were missing) within 1 day after the experiment was initiated,
and this is significantly (P < 0.01) less than the proportion of experiment
I nests at which the cowbird egg disappeared within 1 day (19-1 vs. 5-6).
Events at the remaining five nests were variable. The cowbird egg in
nest 69-228 was ejected after 2-22 days. At nest 69-222 the cowbird
egg remained in the nest for 2-2 days while laying proceeded and the
nest was then predated. The cowbird egg in nest 69-251 was accepted
along with the three catbird eggs subsequently deposited. At nest 72-215
the cowbird egg and the second catbird egg were present the next morning.
This second catbird egg was replaced by a cowbird egg but both cowbird
eggs were missing that afternoon. The two catbird eggs were then returned
to the nest and three catbird eggs were there the next morning.
The complicated events at the last nest (69-264) are worth reporting
in detail: 28 May, first egg laid replaced with an artificial cowbird egg;
29 May, one catbird q- one artificial cowbird egg, replaced the former with
second artificial cowbird egg; 30 May, one catbird egg q- one real (nat-
urally deposited) cowbird egg q- two artifical cowbird eggs, removed the
catbird egg and added a third artificial cowbird egg; 31 May, one catbird
egg q- three artificial cowbird eggs, real cowbird egg is gone, replaced the
catbird egg with a fourth artificial cowbird egg; 1, 2, 3, and 5 June, nest
active, contents four artificial cowbird eggs; 7 June, same, added a real
catbird egg; 8 June, four artificial cowbird eggs q- real catbird egg; 9
June, same; I 1 June, four artificial cowbird eggs, real catbird egg is gone,
added an artificial catbird egg; 13 June, four artificial cowbird eggs q-
one artificial catbird egg; 14 June, same; 15 June, last nest visit, same,
nest still active. This history shows that the catbirds at nest 69-264 ap-
parently accepted a clutch consisting only of artificial cowbird eggs. I
suspect the catbirds removed both the real catbird egg, which I added on
7 June, and the real cowbird egg, which appeared on 30 May.
Thus while the experiment I series shows that some catbirds recognize
their own eggs even in the initial stages of egg-laying, the situation is
rather complex for other individuals. By contrast none of the 29 pairs
of catbirds in eastern North America whose nests received experimental
cowbird eggs when at least two catbird eggs were present (experiment
II), showed comparable complexities. All ejected the cowbird egg within
3 days, although as shown in Table 1, two had not yet done so by the
end of the first 2 days.
EVIDENCE THAT EGG RECOGNITION IS LEARNED
The results (Table 1) suggest that the catbird learns to recognize its
eggs through an imprinting-like process on the first egg or eggs that a
bird sees. The evidence is derived from several aspects. First, catbirds
were relatively tolerant of cowbird eggs that replaced their first egg. The
cowbird egg in four of the 11 nests in the experiment I series was still
accepted after 2 days. This is opposed to only two of 29 for other ex-
periments in eastern North America in which a cowbird egg was added
when there were at least two host eggs in the nest (see experiment II,
Table 1). The difference in 2-day acceptance rates (4-7 vs. 2-27) is sig-
nificant at P = 0.039. If the criterion of a I-day acceptance rate is ap-
plied, the diffference (now 5-6 vs. 4-25) is also significant (at P = 0.047).
It is possible that the longer acceptance time in the experiment I nests
occurred because some of the birds in question had not yet fully learned
their egg type when I removed their first egg. The presence of the cow-
bird egg alone, where previously only a catbird egg had been present, may
have interfered with the egg recognition of some birds and either caused
them to delay their rejection or to accept both egg types.
In contrast to learned egg recognition, two alternative interpretations
can be offered to explain the differences between the results of experiments
I and II. Birds at experiment I nests could have shown increased ac-
ceptance because their motivational state, as regards rejection, was lower
than that of birds at experiment II nests. A number of records indicate
that rejecter species show slightly greater tolerance towards foreign eggs
as their nesting cycle progresses (Rothstein 1970). This increased toler-
ance may occur because the later a cowbird egg appears in the host's
nesting cycle the less chance it has of hatching and of resulting in harm
to the host's own young. On this basis, experiment II rather than experi-
ment I should have had the higher acceptance rate. Secondly, it could
be argued that birds at experiment I nests showed greater acceptance than
those at experiment II nests because they visited their nests less fre-
quently. While catbirds were usually in close attendance to their nests
on the first day of egg laying, my observations indicate that birds at
experiment II nests were in more frequent contact with their nests, so
the possible confounding factor of differential contact with the nest can-
not be completely discounted. However, frequent nest inspections after
egg-laying has begun are to be expected, as this would permit early de-
tection of predation. In addition, while they have greater contact with
their nest, incubating birds may see their eggs less frequently than do birds
early in the egg-laying stage because they keep the eggs covered. Lastly,
evidence presented below supports learned egg recognition, nearly all of
it independent of the problem of differential contact with the nest.
At several nests events occurring more than 2 days after experiment I
was initiated also support the hypothesis of learned egg recognition. At
nest 69-251 the cowbird egg was accepted for at least 8 days before the
nest was destroyed. No other catbirds tested in eastern North America
accepted a single cowbird egg for more than 3 days. Nest 69-228 is also
relevant; here the cowbird egg placed in the nest on the first day of laying
was accepted for 2-21 days, after which the birds continued to incubate
two eggs of their own. Nine days after laying was completed I removed
both catbird eggs and added two artificial cowbird eggs, which the birds
ejected within 6 h, indicating that they were then able to detect this egg
type as foreign quickly whereas earlier they needed at least 2 days.
Learned egg recognition is most strongly supported by the results from
nest 69-264. At this nest, the catbirds accepted a clutch containing only
artificial cowbird eggs when these were deposited in such a manner that
each cowbird egg replaced a catbird egg soon after the latter was laid.
The best explanation for the results from this nest is that the catbird
eggs were not in the nest long enough to allow the birds (or just the fe-
male) to learn this egg type as their own and that they learned the arti-
ficial cowbird egg as "their own type." That they still retained rejection
behavior is indicated by the disappearance of both the naturally deposited
cowbird egg and the real catbird egg that were added to the nest on days
2 and 10, respectively. It seems likely that both these eggs were removed
because they were detectably different from the artificial cowbird eggs.
(Real cowbird eggs show great variation, whereas the artificial ones were
identical mimics of an average cowbird egg.) A catbird nest at Delta,
Manitoba in June 1970 yielded similar data. An artificial cowbird egg
was added to the nest on the day the catbird laid its third or fourth egg.
This cowbird egg was one of the few (three of 55) anywhere in North
America that was accepted when added to a nest already containing more
than one catbird egg; but a naturally deposited cowbird egg appearing
7 days later was ejected while the artificial cowbird egg was left in the
nest. In this case the catbirds may have learned both the artificial cow-
bird egg and the catbird egg patterns as their own and rejected the real
cowbird egg because it was sufficiently different from both these egg types.
The most reasonable interpretation of these results is that the tendency
to reject eggs other than their own is largely innate in catbirds but that
individuals normally learn the egg type responded to as their own from
the first eggs that appear in their nest. Once the individual learns its
egg type, it presumably ejects all other eggs that depart from this type
to a sufficient degree (catbirds will accept some types of nonmimetic
eggs (Rothstein 1970)). Possibly a genetic component in this learning
process prevents catbirds learning any eggs whose features lie beyond a
certain range of variables as their own.
An important question regarding the learning process is whether it oc-
curs only once or with each nesting attempt. If the former is the case the
variation in response to experiment I could be due to some of the birds
being naive and some experienced. If the latter is the case the variation
could be due to the length of time the birds were in contact with their first
egg before I replaced it with a cowbird egg. Since naive and experienced
birds could not be differentiated, my data do not permit resolving this
question definitely. I tested the hypothesis that learning occurs only in
naive birds by determining whether nests in experiment I that yielded
acceptances had smaller eggs, smaller clutches, or were started later than
those that resulted in rejections. Birds breeding for the first time often
have smaller eggs (Romanoff and Romanoff 1949, Coulso.n et al. 1969)
and often have smaller clutches and breed later (Klomp 1970) than older
birds. These analyses failed to demonstrate a correlation between egg
or clutch size or date of laying and response to experiment I, suggesting
that the factor responsible for the variation in response to the experiment
was related to how long the birds were in contact with their first egg be-
fore I started the experiment and not to whether the birds were naive or
experienced. But as I found no correlation between response to experiment
I and time of day I started the experiment, the length of time the birds
were in contact with the first egg was also apparently unimportant. None
of these analyses provides a definite explanation for the variation in re-
sponses to experiment I, but I believe learning probably occurs primarily
in naive birds, and each breeding attempt may further refine it.
If egg recognition is learned, an individual of a rejecter species might
accept a naturally deposited cowbird egg if this egg replaces its own first
egg or is deposited before any of its own eggs. Probably this is a rare oc-
currence, but it seems to have taken place at a catbird nest in the same
study area (New Haven County, Connecticut) and at the same time that
I conducted most of the experiments reported here. On 21 May 1969 I
found an unlined catbird nest. When next visited on 24 May, the com-
pleted nest contained one naturally deposited cowbird egg that a catbird
was incubating. The bird behaved normally in that it scolded me while
I was at the nest. I checked the nest once or twice daily until the morn-
ing of 31 May when it was empty. Until this last visit, the nest con-
tained only the cowbird egg. On all nine visits from 25 May until 30
May (my penultimate visit) the cowbird egg showed signs of being in-
cubated (it was warm), and a catbird scolded me. It is unlikely that
incubation behavior by the catbird would have occurred in the absence
of egg-laying, so the most likely interpretation of the events at this nest
is that the cowbird egg appeared shortly before any catbird eggs or
quickly replaced the first catbird egg (female cowbirds often remove a
host egg (Friedmann 1963)). In either case, the catbird could have
learned the cowbird egg as its own egg type. Then, shortly after laying
each of its own eggs, it may have ejected them since they were unlike the
egg it had learned as its own. The catbird continued to incubate the cow-
bird egg until it was removed, perhaps by a predator, between 30 and 31
May. This explanation accounts for the unique features of this nest--a
cowbird egg was accepted and incubated, and only a cowbird egg, never
a catbird egg, was seen in the nest.
Experiments with another egg type also suggest that egg recognition
is learned. As indicated above, rejecter species generally show slightly
greater tolerance towards foreign eggs as their nesting cycle progresses,
but catbirds show the reverse tendency when parasitized with an egg that
differs only slightly from their own. This egg, type Va, measures 23.32
X 17.91 mm and has a blue-white ground color with 16 symmetrically
placed brown dots (each dot being 0.5 to 1 mm in diameter). Real cat-
bird eggs differ little in size but are blue-green and unspotted. All four
catbirds parasitized with egg type Va after they had completed their
clutch rejected the egg whereas all five parasitized during the egg-laying
period accepted it. This difference (0-4 vs. 5-0) is significant at P < 0.025
and is what one might expect if, as the nesting cycle progresses, learning
continues to refine an individual's recognition behavior so that it becomes
easier for it to detect slight differences. Even if egg recognition is ac-
quired primarily at a bird's first nest, the recognition may be sharpened
temporarily during each successive nesting attempt through prolonged con-
tact with the eggs. Thus, two conflicting tendencies may develop the
longer a bird has contact with its eggs: The drive to reject foreign eggs
decreases, but the ability to detect their presence increases.
Although several independent lines of evidence based on field studies
are all easily explained by the single hypothesis that catbirds learn their
own egg type, this should be confirmed with experiments on breeding
birds of known ages. This could be done most easily in captivity, but
unfortunately none of the North American species that have been found
to reject nonmimetic eggs breed readily in captivity.
EVOLUTIONARY IMPLICATIONS OF LEARNED RECOGNITION
The likelihood that rejecter species learn their egg type and do not
recognize it innately has important evolutionary implications for the
interactions between brood parasites and their hosts. It is commonly
accepted that brood parasites may evolve new egg types that mimic those
of the host because this reduces the host's ability to distinguish between
the parasitic eggs and its own. Some workers have stated that this evo-
lutionary process will eventually end when the mimicry is perfect (Meise
1930 in Southern 1954) and result in "perfect or complete" evolution
(Baker 1942: 3), but the possibility of selective pressures on the host's
eggs has not been given sufficient consideration. It is unlikely that the
egg-related aspects of the evolutionary interactions of a parasite and its
host come to an end and are completed if the parasite's egg mimics the
host's egg perfectly. Selection might favor changes in the host's eggs so
that these diverge in appearance from a highly mimetic parasitic egg. Evo-
lution could take two different pathways. The development of egg poly-
morphism in the host would make it difficult for the parasites to match
their eggs to those of the host even if they had egg types identical to
one or all of the host's morphs (Rothstein 1971, Victoria 1972). This
matching difficulty would arise because brood parasites apparently com-
mit themselves to host nests before any eggs appear (Chance 1940, Hann
1941) and thus have no way of predicting what egg morph a given nest
will contain. Another strategy would be for the hosts to. undergo. direc-
tional selection and evolve a single new egg type as the parasite's mimicry
of their old type becomes more highly developed. Swynnerton (1918)
also suggested that brood parasitism might be a selective pressure that
modified the appearance of host eggs. He stated (1918: 145) "that a
race may in some cases have taken place between the host's eggs and
those of the overtaking Cuckoo"; of course, this race need never end.
While these evolutionary responses by the host to the egg-related adap-
tations of the parasite are a logical expectation, they would have little
chance of occurring if the hosts innately recognized their own egg type.
When new egg variations appeared, the hosts would reject their own eggs,
unless the genetic change producing the new egg type was accompanied by
another genetic change permitting the individual to recognize innately
the new egg type as its own. But, if the egg recognition is not rigidly
determined by innate means and is learned, then the successful develop-
ment of new, more adaptive host egg types would require only the genetic
change that affects the egg's phenotype. Thus hosts with learned egg rec-
ognition should be able to evolve additional antiparasite adaptations much
more rapidly than hosts with innate egg recognition.
Swynnerton (1918) and Victoria (1972) collected data that support
the above arguments. Each worked on a different species of African
weaver (Swynnerton probably Hyphantornis jamesoni and Victoria Plo-
ceus cucullatus). Both species are cuckoo hosts, have highly variable
eggs, and reject nonmimetic eggs. Even the eggs of other members of
their own species are rejected if such eggs are of a sufficiently different
type. The two authors thought it likely that the weavers evolved both
rejection behavior and variable eggs in response to cuckoo parasitism.
Certainly these apparent adaptations of the weavers could have evolved
much more easily if their egg recognition was largely learned rather than
innate. It should be indicated though, that like learned egg recognition,
rejection of discordant eggs would facilitate evolutionary changes in host
eggs. The hosts would always reject only the minority type egg and in
nearly all cases the parasitic egg would be the minority type. But Swyn-
nerton's evidence (1916: 559) suggests, and Victoria's (1972) proves,
that the two weaver species involved actually recognize their own eggs.
If egg recognition via a primarily learned route proves to be the general
mechanism among passerines, it is important from an evolutionary view-
point to ask why it rather than a primarily innate mechanism evolved.
As indicated above, learned recognition would provide advantages in the
future because of its evolutionary flexibility, but it would be teleological
to suggest that these future advantages would enhance the initial develop-
ment of egg recognition by learning. I suggest that learned egg recognition
was simply evolved more easily than innate recognition. Birds are rela-
tively flexible in their behavior and perhaps even among accepter species
older individuals become conditioned to the egg type they have seen in
their many breeding attempts. If in some individuals this predilection
to conditioning were strengthened by genetic determinants, the species
might then evolve into a rejecter species. Possibly even in the beginning
of the evolution of rejection behavior, simultaneous selection for a new
host egg type sometimes facilitates the discrimination between host and
parasitic eggs. Such simultaneous selective pressures might result in the
development of both rejection behavior and learned egg recognition.
ACKNOWLEDGI'vtEN'TS
I thank N. Philip Ashmole, Richard S. Miller, and Charles G. Sibley who aided
me with the thesis on which this paper is partially based. Adrian M. Wennet and
Eugene S. Morton made valuable comments on the manuscript. Larry C. Holcomb
graciously performed some of the experiments cited in this paper. This research
has been suppo.rted by the Frank M. Chapman Memo.rial Fund, Yale University,
the Smithsonian Institution, and the University of California.
SUMMARY
Another paper in this series demonstrated that birds that reject foreign eggs practice true egg recognition after their own clutch is completed and do not simply reject any egg type that is in the minority. This paper attempts to answer the questions of whether this recognition is as highly developed when egg-laying starts and whether it has both learned and innate components. The first egg laid in 11 catbird nests was replaced with an artificial cowbird egg. At six nests the cowbird egg was rejected either before or shortly after the second catbird egg appeared. In the case of these nests the catbirds clearly knew their own egg type at the onset of laying. On an overall basis these 11 nests demonstrated significantly greater tolerance towards cowbird eggs than nests experimentally parasitized later in the breeding cycle. This suggests that a learning component to egg recognition exists. At one nest the catbirds' entire clutch was gradually replaced with artificial cowbird eggs. These were accepted, but a real catbird egg was then rejected, suggesting that the egg type responded to as the catbird's own is learned from the first eggs an individual sees in its nest and that the tendency to reject other eggs is innate. Data from additional experimental and nonexperimental nests also support this hypothesis, but none of the data allow a determination of whether the learning occurs only once in an individual's lifetime at its first nest or repeatedly with each nest. Egg recognition by learning has significant implications as regards the long range evolutionary interactions between brood parasites and their hosts. Hosts that learn their own egg type can evolve additional antiparasite adaptations much more easily than hosts that innately recognize their own egg.
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Department of Biological Sciences, University of California, Santa
Barbara, California 93106. Accepted 17 December 1973.