Fulvous Whistling-Ducks (Dendrocygna bicolor) that nest in rice fields
along the Gulf Coast of southeastern Texas suffered a major population
decline in the late 1960s. This decline was attributed to mortality from
dieldrin (a derivative of aldrin) or aldrin (Flickinger and King 1972).
Although registered for insect control in newly planted rice fields, aldrin
may have been used as an avicide and applied at illegal rates. Numbers
of Fulvous Whistling-Ducks increased in the early 1970s after aldrin
treatment of rice seed was voluntarily discontinued in some nesting hab-
itats (Flickinger et al. 1977, 1980). In 1974 the U.S. Environmental
Protection Agency cancelled the use of aldrin as a treatment for rice seed;
however, Fulvous Whistling-Duck populations have not recovered to the
numbers estimated for Texas in the early 1950s (Singleton 1953).
Concurrently, numbers of Fulvous Whistling-Ducks declined during
the 1960s on their wintering grounds in Mexico; a large 5 state area
extending from Veracruz on the Gulf Coast southward to the Pacific
Coast (Flickinger et al. 1973). However, the literature contains no in-
formation on possible contamination of this species in Mexico. This study
was begun in Texas to determine if Fulvous Whistling-Ducks continue
to carry harmful levels of organochlorine contaminants and if the species
is exposed to contaminants in Texas or Mexico. We determined concen-
trations of organochlorine insecticides in carcasses of adult Fulvous
Whistling-Ducks immediately after spring arrival in Texas and in ju-
veniles before they departed for Mexico in the fall.
METHODS
In spring 1983, adult Fulvous Whistling-Ducks were collected by
shooting on a small reservoir in southern Wharton County between 22
April and 3 May, shortly after the birds arrived from their wintering
grounds. Southern Wharton County is a traditional early spring concen-
tration and nesting area in the southwestern end of the Texas rice belt.
In July, levees along rice fields were searched for nesting ducks to facil-
itate collection of juveniles. Sixteen juveniles from flightless to fledgling
stages were collected from six broods by shooting and by hand at 3
reservoirs near rice fields in Wharton, Colorado, and Jefferson counties
from 3] August to ]] September ]983.
All ducks were weighed and skinned. Feet, bills, wingtips, and gas-
trointestinal tracts were removed. Gastrointestinal tracts were examined
for food content. Each carcass was analyzed at the Patuxent Wildlife
Research Center, Laurel, Maryland for a series of organochlorine con-
taminants: p,p-DDT, p,p-DDD, p,p-DDE, dieldrin, endrin, hepta-
chlor epoxide, oxychlordane, cis-chlordane, trans-nonachlor, cis-nona-
chlor, toxaphene, and polychlorinated biphenyls (PCBs). We used the
analytical method of Cromartie et al. (1975) as modified by Kaiser et al.
(1980).
Residues were identified and quantified by electron-capture, gas-liquid
chromatography (GLC) using a 1.8 m 1.5/1.95% SP-2250/SP-2401
column. Residues in 13 percent of the samples were confirmed with a
Finnigan model 4000 GLC/mass spectrometer. For comparison, average
percent recovery in fortified Mallard (Arias platyrhynchos) tissues ranged
from 76-104%. Residues were not adjusted for percent recovery. The
lower limit of reportable residues was 0.1 ppm for pesticides and 0.5
ppm for PCBs.
In calculating geometric means, a value of one-half the lower limit of
quantification was used for samples in which residues were not detected.
Following an initial 2-way analysis of variance (ANOVA), the Bonfer-
toni multiple comparison method was used to test for significant (P -
0.05) differences in log-transformed carcass residues among years and
between adult males and females. The antilogs (geometric means) are
presented in Table 1.
RESULTS
The arrival of Fulvous Whistling-Ducks in the Texas nesting area in
spring ] 983 coincided with the planting and flooding of rice fields, which
began in the Wharton County study area about 12 April. Planting con-
tinued through 3 May when 447 adult Fulvous Whistling-Ducks were
counted in nearby rice fields. Seven of ] 5 collected adults (47%) had rice
in their gastrointestinal tracts and the carcasses of 5 of the 7 (7]%)
contained dieldrin residues ( -- 0.65 ppm, range 0.11-].9 ppm). In
comparison only 2 of 8 (25%) ducks without rice in their gastrointestinal
tracts contained dieldrin in their carcasses ( --- 3.2 ppm, range 2.0-4.3
ppm). There were no significant differences in geometric means of diel-
drin concentrations between males (0.09 ppm, range 0-0.7 ppm; n = 7)
and females (0.34 ppm, range 0-4.3 ppm; n = 8) shot in 1983 or between
males (0.4 ppm, range 0.1-].6 ppm; n -- 2) and females (0.23 ppm,
range 0-].3 ppm; n -- 4) shot in 1967 or between males (6.35 ppm,
range 2.0-]6.0 ppm; n -- 3) and females (].25 ppm, range 0.5-3.] ppm;
n -- 2) found dead in 1960-]969.
The geometric mean for dieldrin residues in adults (sexes combined)
shot in 1983 was not significantly different from the mean for adults shot
in 1967 (Table 1). However, the former was significantly lower than the
mean for adults found dead in the 1960s. Because of the larger standard
error associated with the mean for dieldrin residues in adults shot in
1967, that mean was not statistically different from the mean for those
found dead in the 1960s.
The 4 adults (27%) in 1983 with the highest dieldrin residues also
contained low levels of endrin. Similar endrin residues were found in 2
of 6 (33%) ducks shot in 1967 and 2 of 5 (40%) found dead between
1967 and 1969 (Table 1). No other organochlorine residues were de-
tected in 1983. Two of 6 adults in the late 1960s contained a trace ((0.1
ppm) of aldrin and 3 contained residues of DDT and its metabolites
(Flickinger and King 1972).
No organochlorine residues were detected in juveniles in 1983. Where-
as in 1967, 3 composite samples of flightless juveniles (10 total) from 3
broods found dead in an aldrin-treated rice field contained dieldrin, en-
drin (Table 1) and DDT and its derivatives. No mortality of adults or
young was observed in rice fields or at reservoirs in 1983.
DISCUSSION
Although we did not demonstrate where or when the adults were
exposed to dieldrin and endrin in 1983, we propose the following expla-
nation. Robinson et al. (1967) found that the mean half-life of dieldrin
in bird tissues was 47 d. Since Fulvous Whistling-Ducks migrate between
the nesting area and wintering grounds in less than 47 d (Flickinger et
al. 1973), adults could have arrived in the nesting area with residues
acquired in Mexico. Yet, the dieldrin residues in ducks in 1983 could
not have been the half-life levels, otherwise some of the ducks would
have died in Mexico from the initial higher residues that would have
been of similar magnitude to those in ducks found dead in Texas rice
fields in the 1960s. Furthermore, White et al. (1982, 1983a,b, 1985)
have shown that birds exposed to organochlorine contaminants on win-
tering grounds in southern Texas (between the rice country and Mexico);
in Veracruz, Mexico; and in Latin America contained more DDE than
dieldrin, but not much of either. Moreover, adults shot in Texas rice
fields in 1983 contained no DDE. However, there has been almost con-
tinuous illegal use of aldrin during rice planting in April and May in
some areas of southeastern Texas since the registration of this chemical
was cancelled for rice seed treatment in 1974 (Peter Nylander, U.S. Fish
and Wildlife Service Special Agent, pers. comm.). Fulvous Whistling-
Ducks have wide movement patterns through the rice country (Flickinger
et al. 1973) and disperse widely after their arrival in Texas. More ducks
that fed on rice seed contained dieldrin residues than ducks which had
not eaten rice. Ducks exposed to contaminants in Texas rice fields in-
variably contained more dieldrin than any other organochlorine residues
(Flickinger and King 1972). We conclude that the collected adults in
1983 were probably exposed to dieldrin and endrin in some nesting areas.
88]
E. L. Flickinger et al.
j. Field Ornithol.
Spring 1986
But, young ducks feeding only in local areas accumulated no residues
indicating that not all nesting environments in Texas are contaminated.
SUMMARY
Fulvous Whistling-Ducks were collected near nesting areas in the rice fields of Texas in 1983 to determine if these birds continue to carry residue levels of organochlorine contaminants, and if the contaminants were acquired in Texas or Mexico. Adults were collected immediately after they arrived from wintering grounds and juveniles were collected in late summer before their departure to wintering grounds. Carcasses of 7 of 15 adults, some of which had been feeding in rice fields, contained residues of dieldrin in low to moderately high levels and 4 contained low levels of endrin. None of 16 flightless juveniles contained organochlorine residues. The contaminated adults were most likely exposed to aldrin or dieldrin via illegal treatment of rice seed with aldrin in some nesting areas of Texas.
ACKNOWLEDGMENTS
Appreciation is extended to Kirby Brown of the Texas Parks and
Wildlife Department for help in collecting juvenile Fulvous Whistling-
Ducks; to Clementine Glenn for typing the manuscript; and to Thomas
Custer, Harry Ohlendorf, and Lawrence DeWeese for editing the manu-
script.
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Coast Field Station, P.O. Box 2506, Victoria, Texas 77902 (ELF, CAM);
Patuxent Wildlife Research Center, Laurel, Maryland 20708 (AJK). Re-
ceived 27 Sept. 1985; accepted 22 Dec. 1985.